Seasonal variation of pollution biomarkers to assess the impact on the health status of juvenile Pacific oysters Crassostrea gigas exposed in situ.

نویسندگان

  • Andrea Luna-Acosta
  • Paco Bustamante
  • Joachim Godefroy
  • Ingrid Fruitier-Arnaudin
  • Hélène Thomas-Guyon
چکیده

BACKGROUND, AIM, AND SCOPE In this study, a suite of sublethal stress biomarkers were analyzed in juveniles of the sentinel species, the Pacific oyster Crassostrea gigas, with a view to using them as pollution monitoring tools. The aim of this work was (1) to study baseline seasonal variations of biomarkers in different body compartments of C. gigas in the reference site and, after selecting biomarkers presenting no seasonal variations, (2) to compare responses of these biomarkers between contaminated and reference sites. MATERIALS AND METHODS Juvenile oysters were transplanted from Bouin (France), a reference site, to three different sites in Marennes-Oleron Bay (France), located in another water body and next to different contamination sources. Animals were exposed in situ for 3 months in summer, autumn, and winter. The following biomarkers were measured: superoxide dismutase (SOD) and glutathione peroxidase (GPx) in gills and digestive gland and lysozyme and phenoloxidase (PO) in plasma. RESULTS No significant seasonal variations for SOD in gills and digestive gland, GPx in gills, and PO in plasma were observed in the reference site. Significant differences in enzyme activity were observed between contaminated and reference sites for SOD in gills and digestive gland and PO in plasma, depending on the body compartment, the season, and/or the site. CONCLUSIONS In conclusion, these data suggest the potential application of these biomarkers in C. gigas to provide ecologically relevant information and, therefore, to be used as biomarkers in coastal pollution monitoring.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Combination of a pesticide exposure and a bacterial challenge: in vivo effects on immune response of Pacific oyster, Crassostrea gigas (Thunberg).

To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to...

متن کامل

Impact of food availability on energy storage and defense related hemocyte parameters of the Pacific oyster Crassostrea gigas during an experimental reproductive cycle

The aim of this study was to test the effect of food quantity on energy storage and defense capacities of oysters during a reproductive cycle. One-year-old Crassostrea gigas oysters were fed two different dietary rations (4% and 12% of oyster dry weight in algal dry weight per day) in controlled experimental conditions over an annual cycle. Oyster dry weights, carbohydrate and lipid contents, e...

متن کامل

Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). II. Response to selection for survival and its influence on growth and yield

Response to divergent selection for “high” and “low” survival during the summer period, from July to October, was investigated in juvenile (six-month-old) Pacific oysters, Crassostrea gigas, by producing two sets of progenies in 2002 (Generation 2) and three sets of progenies in 2003 (Generation 3). A strict between-family approach was used and resistance of these selected progenies to summer m...

متن کامل

Stress and stress-induced neuroendocrine changes increase the susceptibility of juvenile oysters (Crassostrea gigas) to Vibrio splendidus.

Oysters are permanently exposed to various microbes, and their defense system is continuously solicited to prevent accumulation of invading and pathogenic organisms. Therefore, impairment of the animal's defense system usually results in mass mortalities in cultured oyster stocks or increased bacterial loads in food products intended for human consumption. In the present study, experiments were...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Environmental science and pollution research international

دوره 17 4  شماره 

صفحات  -

تاریخ انتشار 2010